rnascope target retrieval reagents Search Results


rnascope target retrieval buffer  (Simport Scientific)
90
Simport Scientific rnascope target retrieval buffer
Whole slide scans of stained patient tissue sections were obtained using a Vectra whole slide scanner. Images were spectrally unmixed, viewed and quantified using QuPath. A & C) A representative whole slide scan of a GIM H. pylori- positive (A) or H. pylori- negative (C) section showing <t>RNAScope</t> ISH probes ‘H. pylori’ and ‘Eubacteria’ to detect H. pylori (green) and non- H. pylori bacteria (white), respectively. IHC staining against E-cadherin (red), Muc5AC (yellow) and Muc2 (turquoise) are also shown. B & D) A higher magnification of panel A and C, respectively. The coloured arrows show the indicated markers. E-H) Quantification of target markers as percentage coverage of tissue from whole slide scans for each patient group for H. pylori (E), Eubacteria (F) Muc5AC (G) and Muc2 (H). Lines between NAT and GAC data points show matched patient pairs. Horizontal bars indicate the median for each indicated patient group. A Mann-Whitney test was used to determine significance where * indicates p <0.05 and ** indicates p <0.01.
Rnascope Target Retrieval Buffer, supplied by Simport Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope target retrieval buffer/product/Simport Scientific
Average 90 stars, based on 1 article reviews
rnascope target retrieval buffer - by Bioz Stars, 2026-03
90/100 stars
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Whole slide scans of stained patient tissue sections were obtained using a Vectra whole slide scanner. Images were spectrally unmixed, viewed and quantified using QuPath. A & C) A representative whole slide scan of a GIM H. pylori- positive (A) or H. pylori- negative (C) section showing RNAScope ISH probes ‘H. pylori’ and ‘Eubacteria’ to detect H. pylori (green) and non- H. pylori bacteria (white), respectively. IHC staining against E-cadherin (red), Muc5AC (yellow) and Muc2 (turquoise) are also shown. B & D) A higher magnification of panel A and C, respectively. The coloured arrows show the indicated markers. E-H) Quantification of target markers as percentage coverage of tissue from whole slide scans for each patient group for H. pylori (E), Eubacteria (F) Muc5AC (G) and Muc2 (H). Lines between NAT and GAC data points show matched patient pairs. Horizontal bars indicate the median for each indicated patient group. A Mann-Whitney test was used to determine significance where * indicates p <0.05 and ** indicates p <0.01.

Journal: bioRxiv

Article Title: The gatekeeper to gastric cancer; gastric microbiota invade the lamina propria in Helicobacter pylori -associated gastric carcinogenesis

doi: 10.1101/2024.04.22.590522

Figure Lengend Snippet: Whole slide scans of stained patient tissue sections were obtained using a Vectra whole slide scanner. Images were spectrally unmixed, viewed and quantified using QuPath. A & C) A representative whole slide scan of a GIM H. pylori- positive (A) or H. pylori- negative (C) section showing RNAScope ISH probes ‘H. pylori’ and ‘Eubacteria’ to detect H. pylori (green) and non- H. pylori bacteria (white), respectively. IHC staining against E-cadherin (red), Muc5AC (yellow) and Muc2 (turquoise) are also shown. B & D) A higher magnification of panel A and C, respectively. The coloured arrows show the indicated markers. E-H) Quantification of target markers as percentage coverage of tissue from whole slide scans for each patient group for H. pylori (E), Eubacteria (F) Muc5AC (G) and Muc2 (H). Lines between NAT and GAC data points show matched patient pairs. Horizontal bars indicate the median for each indicated patient group. A Mann-Whitney test was used to determine significance where * indicates p <0.05 and ** indicates p <0.01.

Article Snippet: RNAScope target retrieval buffer was heated to ∼98°C in Simport Easy Dip staining jars (Simport; M900-12W) in a microwave oven at 100% power.

Techniques: Staining, Bacteria, Immunohistochemistry, MANN-WHITNEY

Whole slide scans were obtained using a Vectra whole slide scanner. Images were spectrally unmixed and viewed using QuPath. A-F) Representative images showing Eubacterial (white) invasion in patients with CG (A-B), GIM (C), H. pylori negative CG (D) and GAC (E&F) (Inset in Panel F shows bacterial cells in a modified Gram stain). E-cadherin (red), H. pylori (green), Eubacteria (white) and DAPI (blue) are shown for visualisation purposes. Arrows indicate the target markers. Eubacterial invasion is highlighted in white ovals for CG and GIM. A rare occurrence of H. pylori invasion to the lamina propria is indicated by the green arrow in (C). G) Representative image showing Eubacterial (white) and Bacillota (yellow) invasion, and H. pylori (green) in a patient with CG. Bacillotas (Firmicutes) were detected using an RNAScope probe against target 16S rRNA sequences. H) Qualitative FISH scoring of Eubacterial invasion for each patient group; 0 = no invasion, 1 = sparse, 2 = moderate, 3 = high and 4 = colonisation. Horizontal bars indicate the mean for each indicated patient group. A Mann-Whitney test was used to determine significance where * indicates p <0.05. Lines between NAT and GAC data points show matched patient pairs.

Journal: bioRxiv

Article Title: The gatekeeper to gastric cancer; gastric microbiota invade the lamina propria in Helicobacter pylori -associated gastric carcinogenesis

doi: 10.1101/2024.04.22.590522

Figure Lengend Snippet: Whole slide scans were obtained using a Vectra whole slide scanner. Images were spectrally unmixed and viewed using QuPath. A-F) Representative images showing Eubacterial (white) invasion in patients with CG (A-B), GIM (C), H. pylori negative CG (D) and GAC (E&F) (Inset in Panel F shows bacterial cells in a modified Gram stain). E-cadherin (red), H. pylori (green), Eubacteria (white) and DAPI (blue) are shown for visualisation purposes. Arrows indicate the target markers. Eubacterial invasion is highlighted in white ovals for CG and GIM. A rare occurrence of H. pylori invasion to the lamina propria is indicated by the green arrow in (C). G) Representative image showing Eubacterial (white) and Bacillota (yellow) invasion, and H. pylori (green) in a patient with CG. Bacillotas (Firmicutes) were detected using an RNAScope probe against target 16S rRNA sequences. H) Qualitative FISH scoring of Eubacterial invasion for each patient group; 0 = no invasion, 1 = sparse, 2 = moderate, 3 = high and 4 = colonisation. Horizontal bars indicate the mean for each indicated patient group. A Mann-Whitney test was used to determine significance where * indicates p <0.05. Lines between NAT and GAC data points show matched patient pairs.

Article Snippet: RNAScope target retrieval buffer was heated to ∼98°C in Simport Easy Dip staining jars (Simport; M900-12W) in a microwave oven at 100% power.

Techniques: Modification, Staining, MANN-WHITNEY